Table 1 PCR primers, including their tags for sample pooling.
From: Metabarcoding of marine environmental DNA based on mitochondrial and nuclear genes
Primer name | Direc-tion | Sequence 5′-3 | Reference |
|---|---|---|---|
COI | |||
mlCOIintR | R | GGRGGRTASACSGTTCASCCSGTSCC | Leray et al.31 |
mlCOIintK | R | GGRGGRTAWACWGTTCAWCCWGTWCC | Present study |
nsCOIFo | F | THATRATNGGNGGNTTYGGNAAHTG | Present study |
Tags | F | actgac, gtagca, gacagt, tgtacg, catctg | Present study |
Tags | R | gacgta, tctgag, gctagt, agcact, agagac | Present study |
18S | |||
18S#4 | F | AGGTCWGTRATGCCCTYMG | Machida & Knowlton 201229 |
18S#5_RC | R | TGYACAAAGGBCAGGGAC C | Machida & Knowlton 201229 |
Tags | F | gtca, catg, acgt, tagc, agac | Present study |
Tags | R | actc, gaga, ctct, gcat, tgag | Present study |
