Figure 3
Quantitative characterization of the behavior of the Blue-OFF system to calibrate the mathematical model. The model was calibrated using kinetic (a) and intensity dose response data (b). For the dose response HEK-293T cells were transfected with the Blue-OFF system. The cells were kept in darkness for 16 h after transfection and subsequently illuminated for 8 h with 0, 2.5, 5, 10, 15 and 20 µmol m−2 s−1 of 460 nm light. Shown is the relative reporter expression on a logarithmic scale. The dots indicate the experimental data points and the solid lines show the model simulation for the optimal parameter set. The shaded error bands are estimated by using an error model assuming a log-normally distributed error.
