Figure 4
Model-aided prediction of protein expression levels using the Blue-OFF system. (a) Simulated reporter expression for different light intensities and illumination times for characterizing the system. To validate if the model can be used to determine experimental conditions to obtain a desired protein level six combinations with different intensities and illumination durations were measured and compared to the model predictions (red circles). The colored filling in the red circles indicates the experimentally determined expression levels. (b) The means of four determinations for each combination are denoted with blue stars and the error bars are showing the standard error of the mean. The colored boxes show the 95% prediction confidence interval calculated by analyzing the prediction profile likelihood.
