Figure 3
From: Engineering functional BMP-2 expressing teratoma-derived fibroblasts for enhancing osteogenesis
Osteogenic efficacy of TDF BMP2/HSV-tk. (A) TDF vehicle control and TDF BMP2/HSV-tk cells were cultured in osteogenic induction medium for 10 days. Morphological changes in TDF BMP2/HSV-tk were examined by light microscopy. The scale bar represents 500 μm. (B) ALP activity was measured in the TDFs treated with or without rh-BMP2 (1, 2, and 5 ng/mL), at days 3 and 7 after the in vitro osteogenesis in osteogenic induction medium. In vitro ALP activity of TDF BMP2/HSV-tk was also determined at the same time and compared with the values for ALP enzyme. Calcium contents of TDF vehicle control and TDF BMP2/HSV-tk were evaluated at day 12 after culturing in osteogenic induction medium. (C) The mRNA levels of osteogenic-related marker genes (ALP, IBSP, Runx2, and Osterix) were measured in TDF and TDF BMP2/HSV-tk treated with either growth medium or osteogenic induction medium. (D) TDF and TDF BMP2/HSV-tk were cultured in osteogenic induction medium or growth medium for 12 days. Mineralization was determined by Alizarin Red S staining. TDF vehicle control was stably introduced in only the vehicle plasmid without any encoding genes. Error bars indicated mean ± SD; *p < 0.05, **p < 0.01, and ***p < 0.001compared with the corresponding control.
