Table 1 Plant disease control efficacy of the Trevesia palmata methanol extract and its partitioned fractions.

From: Identification of novel compounds, oleanane- and ursane-type triterpene glycosides, from Trevesia palmata: their biocontrol activity against phytopathogenic fungi

Treatment

Disease control efficacy (%)

RCB

RSB

TGM

TLB

WLR

BPM

PAN

Methanol extract

60 ± 0*

0

79 ± 0**

43 ± 0*

3 ± 4

0

25 ± 11

n-Hexane layer

30 ± 14

0

7 ± 10

7 ± 10

13 ± 10

0

58 ± 12*

Ethyl acetate layer

70 ± 0*

5 ± 7

86 ± 0**

57 ± 0**

3 ± 4

0

50 ± 0*

n-Butanol layer

60 ± 14*

0

75 ± 6**

64 ± 10**

53 ± 0**

0

8 ± 11

Water layer

0

0

0

0

0

0

8 ± 11

Positive control

100**

100**

100**

100**

100**

100**

86 ± 0**

  1. Methanol extract and each fraction (2,000 μg/ml) were applied onto plants one day prior to inoculation with fungal pathogens. As a positive control of each plant disease, blasticidin S (50 μg/ml) for RCB, validamycin (50 μg/ml) for RSB, fludioxonil (50 μg/ml) for TGM, dimethomorph (10 μg/ml) for TLB, flusilazole (10 μg/ml) for WLR, flusilazole (10 μg/ml) for BPM, and dithianon (50 μg/ml) for PAN were used. Control value (%) represent the mean ± standard deviation of two runs with three replicates. Asterisks indicate significant difference compared with negative controls by Tukey’s test (**p < 0.001, *p < 0.01). RCB, rice blast (caused by Magnaporthe oryzae); RSB, rice sheath blight (caused by Rhizoctonia solani); TGM, tomato gray mold (caused by Botrytis cinerea); TLB, tomato late blight (caused by Phytophthora infestans); WLR, wheat leaf rust (caused by Puccinia triticina); BPM, barley powdery mildew (caused by Blumeria graminis f. sp. hordei); and PAN, pepper anthracnose (caused by Colletotrichum coccodes).
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