Figure 5
Scolopendrasin IX inhibits inflammatory cytokine production as well as neutrophil recruitment into the synovium joint area in the K/BxN serum-transfer arthritis model. (A) On day 10 after initiation of arthritis, cells in the synovial fluid from each group were stained with anti-CD11b and anti-Ly6G, and neutrophils (CD11b+, Ly6G+) were analyzed by flow cytometry (left). Data are shown in several concentrations (0 mg/kg, 1 mg/kg, 5 mg/kg, 10 mg/kg) of scolopendrasin IX (right). (B) Ankle joint tissues from sham and K/BxN serum-induced arthritic mice injected with vehicle or scolopendrasin IX (1 mg/kg) were homogenized on day 10, and the cytokine levels were measured by ELISA. Data are presented as mean ± SD (n = 5 for A right, B). Data are representative of five mice per group (A left). Data in panels are representative from two independent experiments (A right, B). *p < 0.05, **p < 0.01.
