Figure 1 | Scientific Reports

Figure 1

From: Cytometry meets next-generation sequencing – RNA-Seq of sorted subpopulations reveals regional replication and iron-triggered prophage induction in Corynebacterium glutamicum

Figure 1

Heterogeneous prophage induction in C. glutamicum populations. For proof-of-concept studies, prophage induction was triggered by counter silencing as described previously20. Phage induction was visualized by the means of fluorescent protein production using a fusion of the Plys phage promoter to eyfp. (A) Growth of the strain C. glutamicum::Plys-eyfp/pAN6_N-cgpS treated with different IPTG concentrations. (B) Flow cytometry analysis after six hours revealed a significant fraction of prophage induced cells upon addition of >100 µM IPTG. (C) Contour plots of an induced (blue) and an uninduced sample (red). Gating strategy applied for the isolation of 106 cells from phage positive and negative populations are shown in the right plot.

Back to article page