Figure 3 | Scientific Reports

Figure 3

From: Cytometry meets next-generation sequencing – RNA-Seq of sorted subpopulations reveals regional replication and iron-triggered prophage induction in Corynebacterium glutamicum

Figure 3

Differential gene expression analysis of prophage induction in C. glutamicum. C. glutamicum::Plys-eyfp/pAN6_N-cgpS cells were cultivated in CGXII medium with 2% (w/v) glucose and either with or without 150 µM IPTG. (A) Flow cytometry data show the activation of the phage reporter (blue), compared to the uninduced control (red). (B,C) From both samples RNA was extracted (without cell sorting) and analysed by RNA-Sequencing. The Log2(foldchange) was plotted against the coding regions (orfs) of the C. glutamicum genome. (D) Fluorescence-activated cell sorting to separate induced from uninduced cells from a heterogeneous population. (E,F) Differential gene expression analysis of the induced versus the uninduced subpopulation (see also Table S2). (G) Comparison of expression values of the sorted samples versus the unsorted reference data set (R2 = Pearson correlation coefficient).

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