Figure 6 | Scientific Reports

Figure 6

From: Cytometry meets next-generation sequencing – RNA-Seq of sorted subpopulations reveals regional replication and iron-triggered prophage induction in Corynebacterium glutamicum

Figure 6

Iron triggered prophage induction in C. glutamicum. Shown is a differential gene expression analysis of subpopulations isolated from an iron upshift experiment. C. glutamicum/pJC1_PdviS-venus cells were first cultivated in CGXII medium with 1 µM iron and then shifted to medium with 36 µM iron. (A) Based on the output of the divS promoter fusion, 106 cells of each subpopulation were sorted and treated according to the established RNA-Seq workflow. (B) Differential gene expression analysis: As expected, several SOS genes showed an upregulation in the divS+ subpopulation. Remarkably, also the expression of genes located within the CGP3 prophage element (red dots) were significantly increased indicating iron-triggered prophage induction.

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