Table 1 Strains and plasmids used in this study.

   C. glutamicum ATCC 13032

Biotin-auxotrophic wild type

⁴⁸

   C. glutamicum::Plys-eyfp

Derivative of ATCC 13032 containing the integrated prophage reporter P_lys-eyfp into the intergenic region of cg1121-cg1122

²⁰

   C. glutamicum ΔdtxR

In-frame deletion of the dtxR gene

³⁷

   C. glutamicum ΔdtxR::Plys-eyfp

Derivative of the ΔdtxR strain containing the integrated prophage reporter P_lys-eyfp

This study

   C. glutamicum ΔrecA::Plys-eyfp

In-frame deletion of recA, containing the integrated prophage reporter Plys-eyfp

¹⁸

   E. coli DH5α

supE44 ΔlacU169 (ϕ80lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

Invitrogen

   pAN6

Kan^R; C. glutamicum/E. coli shuttle vector for gene expression under control of the tac promoter; (Ptac, lacI^q, pBL1 oriV_C.g., pUC18 oriVE.c.)

¹⁷

   pAN6-N-cgpS

Kan^R; pAN6 derivative containing the first 65 amino acids of the cgpS gene

²⁰

   pAN6-dtxR

Kan^R; variant of pAN6. Contains dtxR gene under tac promoter for complementation studies.

This study

   pAN6-recA

Kan^R; variant of pAN6. The recA gene was cloned under the influence of the tac promoter (for complementation).

This study

   pJC1

Kan^R; E. coli/C. glutamicum shuttle vector (pHM1519 ori_Cg, pACYC177 ori_Ec)

⁴⁹

   pJC1-venus-term

Kan^R; pJC1 derivative containing the venus gene and additional terminators

⁵⁰

   pJC1-PdivS-venus

Kan^R; pJC1 derivative containing the venus gene under the divS promoter control

This study

   pK18mobsacB-cg1121/1122-P_lys-eyfp

Plasmid for integration of the lysin promoter (of cg1974) fused to eyfp into the intergenic region of cg1121-cg1122

¹⁸