Table 1 Primer sequence for Real-Time PCR analysis.

From: Single Dose Administration of Taheebo Polyphenol Enhances Endurance Capacity in Mice

Genes

Forward

Reverse

GS

ACTGCTTGGGCGTTATCTCTGTG

ATGCCCGCTCCATGCGTA

GP

TGGCAGAAGTGGTGAACAATGAC

CCGTGGAGATCTGCTCCGATA

HK2

CTGTCTACAAGAAACATCCCCATTT

CACCGCCGTCACCATAGC

G6Pase

GTGGCAGTGGTCGGAGACT

ACGGGCGTTGTCCAAAC

PEPCK

CACCATCACCTCCTGGAAGA

GGGTGCAGAATCTCGAGTTG

HADH

ACTACATCAAAATGGGCTCTCAG

AGCAGAAATGGAATGCGGACC

MCAD

GCTCGTGAGCACATTGAAAA

CATTGTCCAAAAGCCAAACC

ACO

TGTTAAGAAGAGTGCCACCA

ATCCATCTCTTCATAACCAAATTT

CPT1Ī±

CCAGGCTACAGTGGGACATT

GAACTTGCCCATGTCCTTGT

CPT1Ī²

CCCATGTGCTCCTACCAGAT

CCTTGAAGAAGCGACCTTTG

CPT2

GAAGAAGCTGAGCCCTGATG

GCCATGGTATTTGGAGCACT

ACC1

ATTGGGCACCCCAGAGCTA

CCCGCTCCTTCAACTTGCT

ACC2

GGGCTCCCTGGATGACAAC

TTCCGGGAGGAGTTCTGGA

MCD

ACTCCATCAGCCTGACCCAG

ACCCCTTGAGGCTCTCGTGA

PGC-1Ī±

GACTGGAGGAAGACTAAACGGCCA

GCCAGTCACAGGAGGCATCTTT

SIRT1

GCAACAGCATCTTGCCTGAT

GTGCTACTGGTCTCACTT

Cyto c

CACGCTTTACCCTTCGTTCT

CTCATTTCCCTGCCATTCTC

18S

CGGCTACCACATCCAAGGA

AGCTGGAATTACCGCGGC

  1. GS; glycogen synthase, GP; glycogen phosphorylase, HK2; hexokinase 2, G6Pase; glucose-6-phosphatase, PEPCK; phosphoenolpyruvate carboxykinase, HADH; hydroxyacyl-CoA dehydrogenase, MCAD; medium chain acyl-CoA dehydrogenase, ACO; acyl-CoA oxidase, CPT; carnitine palmitoyltransferase, ACC; acetyl-CoA carboxylase, MCD; malonyl-CoA decarboxylase, PGC-1Ī±; peroxisome proliferator-activated receptor gamma coactivator-1 alpha, SIRT1; sirtuin 1, Cyto c; cytochrome c, 18ā€‰S; 18ā€‰s ribosomal RNA.
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