Figure 3

(a) HPLC separation curve of 0.8 mg/ml solution of racemic thalidomide in hexane with ethyl acetate, obtained with (S)-(+)-2-pyrrolidinemethanol-modified 3D GNS stationary phase (MOD3 3D GNS mixed with graphite powder). The inset shows the corresponding polarimetry result (negative angle of polarization rotation) from a flush chromatography test. (b) HPLC separation result (baseline separation) for 0.05 mg/ml solution of racemic thalidomide achieved in an experiment with TCNEO-modified 3D GNS stationary phase (MOD1 3D GNS mixed with graphite powder).