Figure 2
(A,B) NMR spectroscopy of urine samples. (A) Overlay of a 1H 1D CPMG experiment (grey) and the projection of a 1H J-resolved 2D experiment (black) of a urine sample after sugar intake. Both experiments show NMR signals of all metabolites harboring non exchangeable protons. By resolving the J-coupling in the indirect dimension, the spectral signatures are simplified. (B) Projections of 1H J-resolved 2D experiments of reference compounds sucrose (orange), lactulose (magenta), and mannitol (light blue) (each 100 µM). Corresponding resonances of these sugars in a human urine sample before (grey) and after sugar intake (marine blue) are indicated with orange hash (sucrose), magenta circle (lactulose) or light blue asterisks (mannitol).
