Figure 5
From: Influence of ylHog1 MAPK kinase on Yarrowia lipolytica stress response and erythritol production

Western blot analysis of whole cell extracts isolated from Y. lipolytica wild type (MK1), yl-hog1∆ and yl-HOG1 cells after treatment with 1 M NaCl for the specified times. The active form of protein (Hog1-P) was detected using an anti-phospho p38 antibody (upper panel). Total levels of yl-Hog1 protein were determined by probing the blot with an anti-Hog1 antibody (lower panel). The full-length blots are presented in Supplementary Fig. 1.