Figure 1 | Scientific Reports

Figure 1

From: Exosomes from normal and diabetic human corneolimbal keratocytes differentially regulate migration, proliferation and marker expression of limbal epithelial cells

Figure 1The alternative text for this image may have been generated using AI.

Characterization of normal and DM human LSC-derived Exos. (a) Representative TEM images showing a range of exosomal size from 50–200 nm and typical doughnut shape morphology in both N (n = 3) and DM (n = 3) human LSC-derived Exos. (b) size distribution of LSC-derived EVs was determined by NanoSight LM10. Histogram shows particle size distribution typical of exosomes. (c) Flow cytometry was performed on normal (healthy)- and DM-EVs using anti-CD63-coated beads. Vesicles were immunostained against CD63 (red) and CD81 (red) and compared with appropriate isotype control (blue), n = 3. (d) Western blot showed expression of typical exosomal markers HSP70 and CD63 in both N (n = 3) and DM (n = 3) vesicles.

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