Figure 2 | Scientific Reports

Figure 2

From: Exosomes from normal and diabetic human corneolimbal keratocytes differentially regulate migration, proliferation and marker expression of limbal epithelial cells

Figure 2The alternative text for this image may have been generated using AI.

Dil-labeled normal human LSC-derived EVs can be internalized by human primary LEC, HCEC and organ-cultured corneas. Dil-labeled LSC-derived Exos (25 μg/ml or 3 × 108/ml) were incubated (direct addition of Dil-labeled Exos) with organ-cultured corneas, primary LEC and HCEC for 24 h, no Exo was added to the control. Dil-labeled normal keratocytes were co-cultured up to 48 h on trans-wells. The cells were stained with calcein-AM, which exhibits green fluorescence and demonstrates live cells and their uptake of EVs.

Back to article page