Figure 3 | Scientific Reports

Figure 3

From: Exosomes from normal and diabetic human corneolimbal keratocytes differentially regulate migration, proliferation and marker expression of limbal epithelial cells

Figure 3The alternative text for this image may have been generated using AI.

Normal EVs increase migration and proliferation of normal primary LEC. (a) LEC were scratch-wounded and incubated with 25 μg/ml N/DM LSC-derived EV, and wound closure was quantified using ImageJ software at 12 and 24 hr after wounding. Cell migration and wound closure were significantly enhanced in normal primary LEC treated with N-Exos compared to the cells treated with DM-Exos or PBS/untreated control cells. DM-Exos treatments did not change the wound healing rate compared to control. (b) MTS proliferation assay showed increase in proliferation rate in LEC treated with N-Exos vs. those treated with DM-Exos or untreated control cells. DM-Exo treatments did not change the proliferation rate compared to control. The bar graph represents average ± SEM of pooled values of three independent triplicate assays and compared to untreated control cells (negative control). **p < 0.01, *p < 0.05 by paired two-tailed t test.

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