Figure 5

Effect of N and DM Exos on the expression of putative LESC marker, K15, in normal and DM primary LEC. Both normal and DM LEC treated with N-Exos increased K15 expression in comparison with untreated control or DM-Exo treated cells. DM-Exo treated cells showed no change in K15 expression level in comparison to control cells. IgG2aκ isotype control antibody (Thermo Fisher Scientific) was used as negative control. The experiments were performed at least in triplicate and same exposure time was used for each set of compared stained sections, and the assessment was done by more than one observer.