Figure 6
From: Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface
Scanning electron microscopic assessment of ultrastructural changes in BBEC cultures over time. Bronchial epithelial cell cultures were grown until 42 days post-ALI and samples fixed at three day intervals and processed for SEM (see also Fig. S6). Representative micrographs show (i) the undifferentiated, flattened apical surface at day 0 post-ALI with extruding nuclei (arrowheads); (ii) microvilli (arrow) and microplicae (arrowhead) on undifferentiated cells at day 0 post-ALI; (iii) longer microvilli (arrowheads) on day 3 post ALI cells undergoing differentiation; (iv) early stages of cilia formation (arrowheads) on day 6 post-ALI cells undergoing differentiation; (v) numerous well-formed cilia on day 12 post ALI cells; (vi) maximum ciliation on day 24 post-ALI cells; (vii) goblet cells (arrowheads) present in day 21 post-ALI cultures; (viii) section of day 18 post-ALI epithelium showing pseudostratified structure; and (ix) microvilli (arrowheads) at base of cilia associated with day 36 post-ALI cells.
