Figure 3
From: Heat-stress triggers MAPK crosstalk to turn on the hyperosmotic response pathway
Heat-shock activation of HOG depends on the Cell-Wall Integrity pathway. (A) HOG MAPK activation dynamics in WT and Δslt2 cells grown in SC or in SC + 1 M sorbitol following a shift from 30 °C to 37 °C. Left: representative blot. Uncropped blot in Fig. S3. Right. Hog1pp abundance. (B) HOG transcriptional reporter dynamics in WT and Δslt2 grown in SC + 1M sorbitol following a shift from 30 °C to 37 °C. (C) Scatter plot comparing data at time 0 with 120 minutes after temperature shift from 30 °C to 37 °C. (D) CWI MAPK activation dynamics following a shift from 30 °C to 37 °C in cells grown in SC or in SC + 1 M sorbitol. Slt2 phosphorylation was assayed by immunoblotting. Uncropped blot in Fig. S8. (E) HOG transcriptional reporter in the temperature sensitive mutant pkc1–3 measured 2 h after 1 M sorbitol-adapted cells were shifted from 24 °C to 37 °C. (F) HOG transcriptional reporter in yeast stimulated with 50 μg/ml of congo red or 50 μg/ml of calcofluor white. In A, B, E and F values correspond to the mean of three independent replicates ± SEM. Strains: LD3342, RB3376, YPD6020 and YPD6021.
