Figure 5
In vivo suppressive effect of luteolin on IL17A secretion and NLRP3 expression activities. (A) Experimental timeline for DSS-induced colitis and the subsequent oral feeding of different phytochemicals in test mice (B) Western blotting for expression of NLRP3 proteins, in response to in vivo treatment with LAW (0.5 mg/kg) or sulfasalazine (100 mg/kg) in mouse colon tissues. (C) ELISA for secretion of IL-17A in response to in vivo treatment with LAW or sulfasalazine. Effect on secretion of IL-17A for each phytochemical-treated group was compared with that of the DSS-treated group. (D) Effects of LAW or sulfasalazine on the population change in Th17 cells (CD4+IL-17A+) in colon tissues of test mice were quantified by flow cytometry analysis, and were compared with that of the DSS-treated group. Data are reported as the mean of 3 biological repeats. *P < 0.05; **P < 0.01; NS, not significant (2-tailed t-test).
