Figure 5
From: An Evolutionarily Conserved Mesodermal Enhancer in Vertebrate Zic3
Deletion analysis of Zic3-ME in chicken electroporation assay. (A) Mapped Zic3-ME core region and the deletion constructs used for the assay. Right columns indicate the frequencies of the reporter expression in somite or neural tube. Segment 1 and segment 2 indicate the regions, of which deletion abolished the somite expression. (B) Representative results of the deletion assay. Top, bright field view; middle, reporter GFP signals; bottom, β-galactosidase activity staining to indicate the transfected cells. Scale bar, 1 mm.
