Table 1 Coupling efficiencies of DNA phosphoramidites. Left.
From: High-Efficiency Reverse (5′→3′) Synthesis of Complex DNA Microarrays
Phosphoramidites | Coupling efficiency (%) | Phosphoramidites | Coupling efficiency (%) |
|---|---|---|---|
5′ NPPOC | 5′ BzNPPOC | ||
dA | 99.9 | dA | 99.9 |
dC | 99.3 | dC | 99.9 |
dG | 97.4 | dG | 97.1 |
dT | 99.9 | dT | 99.9 |
3′ NPPOC | 3′ BzNPPOC | ||
dA | 99.9 | dA | 99.4 |
dC | 99.0 | dC | 98.8 |
dG | 93.9 | dG | 97.6 |
dT | 99.9 | dT | 99.9 |
