Figure 8

Effects of heat stress on the expression of wnt-1, ash1, ash2, ash3, ase, dll and dpp, srf, salm ser in Rc (black) and Sm (white) pupa DBM. Abscissa: temperature (°C)-treated time (h). Ordinate: relative quantity expression of the genes. Pupae newly formed at 25 °C from both Rc and Sm populations were incubated by four temperature treatments, that is, 25 or 44 °C for 1 h (25–1 or 44–1), 25 or 40 °C for 8 (25–8 or 40–8) or 16 h (40–16), 25 or 42 °C for 4 (25–4 or 42–4) or 8 h (42–8), and 25 or 38 °C for 48 h (25–48 or 38–48), respectively. Living pupae after heat stress were allowed to recovery for 1 h at 25 °C before they were used for extracting mRNA. The values in each group of the four temperature treatments were used for statistical analysis, respectively, in Rc and Sm DBM. Lower-case letter indicates significant difference in mRNA expression in each temperature treatment group (Duncan test, P ≤ 0.05).