Table 3 Sequences of primers used for cloning wing development-related genes cDNAs of DBM.
Names of primers | Sequences of Primers (5′–3′) | Product size(bp) | Names of primers | Sequences of Primers (5′–3′) | Product size(bp) |
|---|---|---|---|---|---|
For initial fragment(s) | For RACE | ||||
Ash1-F | 5′–GGAGGAACGCCAGGGAG–3′ | 3′Ash1-F1 | 5′–CGGTTGTTCAGAGTCGCA–3′ | ||
Ash1-R | 5′–GTTGTTGCCACCACGATA–3′ | 401 | 3′Ash1-F2 | 5′–CCCACCTGTCGAGACACCTA–3′ | 336 |
Ash2-F | 5′–ACTACAACTCGCCCAAGGTCA–3′ | 5′Ash1-R1 | 5′–CAGGGCTCATTGGTTCATAGG–3′ | ||
Ash2-R | 5′–CGGAATAGGCGGAGGACA–3′ | 593 | 5′Ash1-R2 | 5′–TGACACAGCCGAGGGGATG–3′ | 309 |
Ash3-F | 5′–GGGAGAGGAACCGAGTG–3′ | 3′Ash2-F1 | 5′–GAGAGGAACCGAGTGAAGCAAGT–3′ | ||
Ash3-R | 5′–GTACTCCACCACCATGCGG–3′ | 155 | 3′Ash2-F2 | 5′–GGAGAACATCCCCAACGGC–3′ | 287 |
Ase-F | 5′–ACATACCTGAGGAAGTCGC–3′ | 5′Ash2-R1 | 5′–CTGCCGTAGAATAACCCGTC–3′ | ||
Ase-R | 5′–CTTCTCCTGCCACCATTT–3′ | 800 | 5′Ash2-R2 | 5′–ACTTGCTTCACTCGGTTCCTC–3′ | 321 |
Dll-F | 5′–GAATACAAACCCCCGAC–3′ | 3′Ash3-F1 | 5′–GCCTTACCAAACCCTACCTG–3′ | ||
Dll-R | 5′–ATGGTCCTGGGCTTCCTC–3′ | 507 | 3′Ash3-F2 | 5′–ACCGAGTGAAGCAAGTGAACGACG–3′ | 679 |
DPP-F | 5′–GGGATGGAAGCAGGGTGATG–3′ | 5′Ash3-R1 | 5′–GTACTCCACCACCATGCGG–3′ | ||
DPP-R | 5′–GTCCTTTGGAACGGGAGCAG–3′ | 418 | 5′Ash3-R2 | 5′–ATGCGGAGCGTGTCCACCTTGC–3′ | 466 |
Salm-F | 5′–GCGGATGAACTGGCATTTAG–3′ | 3′Ase-F | 5′–TACACGTGCAGATGAACCACAACT–3′ | 725 | |
Salm-R | 5′–CGCCGACGGTTTACTGAGAT–3′ | 1414 | 5′Ase-R1 | 5′–TGGACCACCGTCTTCCTCTT–3′ | |
Ser-F | 5′–CGGTCAGGTCCCGATTA–3′ | 5′Ase-R2 | 5′–GTATTCGACAGCCATGCGTAGT–3′ | 599 | |
Ser-R | 5′–AGGAACCCACCGTAGCG–3′ | 1196 | 3′Dpp-F1 | 5′–TCAACCCGTACCACTACAAGCG–3′ | |
SRF-F | 5′–CCCACCATCCAACGGCAA –3′ | 3′Dpp-F2 | 5′–AACCCGTACCACTACAAGCGA–3′ | 564 | |
SRF-R | 5′– GTCGGGCGAGTTGAGGCA–3′ | 274 | 5′Dpp-R1 | 5′–TCCAGTTCCTCAATAGCCCC–3′ | |
Wnt-l-F | 5′–CCGACGCTGGAACTGCTC–3′ | 5′Dpp-R2 | 5′–CTTCACCAGACTGTCCACCG–3′ | 1155 | |
Wnt-l-R | 5′–ACCTTCTCCGTGTGGCAG–3′ | 880 | 3′SRF-F1 | 5′–CAAGCGGAAGACAGGGATT–3′ | |
For ORF | 3′SRF-F2 | 5′–GCAACACGCAAACTCCAGC–3′ | 594 | ||
Ash1-ORF-F | 5′-GACTCTACACTCGCGATG-3′ | 5′SRF-R1 | 5′–GCTGGAGTTTGCGTCTTGC–3′ | ||
Ash1-ORF-R | 5′-CAAAGCTCCGATACATGATAC-3′ | 712 | 5′SRF-R2 | 5′–AATCCCTGTCTTCCGCTTG–3′ | 268 |
Ash2-ORF-F | 5′-GCGACCGCCACTCACCC-3′ | 5′Wnt-l-R1 | 5′–AGCCGCCCCACTTCCACACGC–3′ | ||
Ash2-ORF-R | 5′-TGTTATTTCTGTTGCCACCACG-3′ | 721 | 5′Wnt-l-R2 | 5′–CGCTCGTGATGGCGTAGATG–3′ | 443 |
Ash3-ORF-F | 5′-AACCCTACCTGTTTACGA-3′ | ||||
Ash3-ORF-R | 5′-CAATGTTTACACAATGCCA-3′ | 860 | UPM*(long) | 5′-CTAATACGACTCACTATAGGGCAAGCAGTGGTAACAACGC | |
Ase-ORF-F | 5′-GCGACTAAACCAAAACAAACACC-3′ | AGAGT-3′ | |||
Ase-ORF-R | 5′-CGTAACTTAGAGGACAAACC-3′ | 1513 | UPM*(short) | 5′-AAGCAGTGGTAACAACGCAGAGT-3′ | |
Dpp-ORF-F | 5′-CGATGGTGGATTAGGCA-3′ | NUP* | 5′-AAGCAGTGGTAACAACGCAGAGT-3′ | ||
Dpp-ORF-R | 5′-TGCGGCTAAGATACTGAGGAG-3′ | 1374 | |||
SRF-ORF-F | 5′-GACCAGTGCTACGACGAG−3′ | ||||
SRF-ORF-R | 5′-GTCCCGAACCGAAAACCT-3′ | 773 | |||
