Table 1 Candidate reference genes and target genes description, primer sequences, and amplicon characteristics in this study.

From: Selection of suitable reference genes for qRT-PCR normalisation under different experimental conditions in Eucommia ulmoides Oliv

Gene symbol	Gene description	Accession number	Primer sequence (5′-3′)	Size (bp)	PCR efficiency (%)	Regression Coefficient (R²)	Tm (°C)
Reference genes
18S rRNA	18S ribosomal RNA	MH890464	CCCCGACTGTTCCTGTTAAT	59	105.5	0.995	77.5
18S rRNA	18S ribosomal RNA	MH890464	TGCGTTTCTATTTTGTTGGTTT	59	105.5	0.995	77.5
ACT	actin	MH890466	GTGTTATGGTTGGGATGGG	108	100.2	0.996	80
ACT	actin	MH890466	TGCTGACTATGCCGTGTTC	108	100.2	0.996	80
ACT97	actin 97	MH890465	CGGGCAGGTCATCACTATCGG	200	97.7	0.997	86.9
ACT97	actin 97	MH890465	CGGCAATCCCAGGAAACATCG	200	97.7	0.997	86.9
HIS2B	Histone H2B	MH890468	GGAAGAAATTGCCAAAGGATG	106	89.1	0.996	81.8
HIS2B	Histone H2B	MH890468	TGCTTGAGGACCTTGAAGATGTA	106	89.1	0.996	81.8
HIS4	histone H4	MH890467	GGGACAACATCCAGGGAATC	160	99.7	0.995	87
HIS4	histone H4	MH890467	GCGTGCTCGGTGTAGGTGA	160	99.7	0.995	87
TUA	alpha-tubulin	MH890463	CATTTCCTCTTTGACTGCCTCC	185	102.8	0.992	84
TUA	alpha-tubulin	MH890463	ATGCGGTGTTGGTGATTTCG	185	102.8	0.992	84
TUB	beta-tubulin	MH890469	AAATGAGCACCAAGGAGGTG	119	100.6	0.991	82.6
TUB	beta-tubulin	MH890469	GGTTGGAGGAATATCGCAGA	119	100.6	0.991	82.6
UBA52	ubiquitin-60S ribosomal protein L40	MH890471	GGCCAGGAAATACAACCAAG	120	90.4	0.995	84
UBA52	ubiquitin-60S ribosomal protein L40	MH890471	TTCTTCGGCCTCAACTGATT	120	90.4	0.995	84
UBA80	ubiquitin extension protein	MH890472	GACCTACACCAAGCCGAAGA	114	105.9	0.992	83.9
UBA80	ubiquitin extension protein	MH890472	CACTCCTTCCTCAGCCTCTG	114	105.9	0.992	83.9
UBC	ubiquitin conjugating enzyme S	MH890470	AGTGGGTGGTGCTGTAGTCC	121	102.5	0.992	82.7
UBC	ubiquitin conjugating enzyme S	MH890470	AACTCCCGTTTCGTTTGTTG	121	102.5	0.992	82.7
UBC E2	ubiquitin-conjugating enzyme E2	MH890473	CAGTGGAGCCCTGCCCTTACC	110	106.4	0.995	82
UBC E2	ubiquitin-conjugating enzyme E2	MH890473	GCGATCTCTGGCACCAACGGG	110	106.4	0.995	82
Target genes
MK	mevalonate kinase	MH890474	GCCGATGAATCACAGAAA	181	105.3	0.993	82.5
MK	mevalonate kinase	MH890474	GCAACGGTGGTGGTAGTA	181	105.3	0.993	82.5
PAL	phenylalanine ammonia-lyase	MH890475	CGGTTTGCCGTCGAATCTGT	74	107.6	0.99	82.5
PAL	phenylalanine ammonia-lyase	MH890475	TCGCTATCTCCGCCCCCTTA	74	107.6	0.99	82.5
4CL	4-coumarate-CoA ligase	MH890476	CGGTGCCTCTGAATCTGCT	143	106.4	0.997	85.5
4CL	4-coumarate-CoA ligase	MH890476	GATGTGGTGCTCTGCGTGC	143	106.4	0.997	85.5

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