Figure 3
Phagocytosis analysis by flow cytometry. (a) Dot plots and histograms showing the CD45int/CD11bhigh double-labelled population corresponding to microglia and the CD45int/CD11bhigh/eGFP+ triple-labelled population representing microglia that have phagocytosed parasites. Left and right panels show microglia alone and microglia co-incubated with parasites for 6 h, respectively. (b) Percentage of triple-positive microglia after 3 h of co-incubation at 37 °C and 4 °C, respectively. The microglia to parasites ratios were 1:5, 1:10, and 1:30, respectively. (c) Time-dependence of phagocytosis evaluated at a 1:30 ratio and 37 °C in co-cultures, in which unbound parasites or detached microglia were washed out. (d) Bars illustrate the percentage of triple labelled cells in supernatants from cultures in C (mean ± S.D. of three individual cultures measured at each condition). The data were processed using the FlowJo software (FlowJo, LLC, Ashland, Oregon, USA). Asterisks represent significant differences, p < 0.001 (***) and p < 0.05 (*).
