Figure 2
From: TRSP is dispensable for the Plasmodium pre-erythrocytic phase
Infectivity of trsp knockout sporozoites in the mammalian host following intravenous or intradermal infection. Infectivity of TRSP- (clones I21 or I26) and control sporozoites following infection by intravenous (A–D) or intradermal (E–H) routes. In these representative experiments, intravenous infections were carried out using 1 × 104 sporozoites, while intradermal inoculations were performed with 1.5–2 × 104 sporozoites. Parasite burden in the liver (n = 3 or 4) was assessed by live imaging on days 1 (D1) and 2 (D2) post-infection using IVIS Lumina LT. The bioluminescent signal as average radiance (photons/s/cm2/sr) of individual mice (symbols) and the average +SD (bars) of groups were plotted (A,C,E,G). Starting on day 3 post-infection, parasitaemia of infected animals was determined daily by a Giemsa-stained blood smear. Parasitaemia of individual mice (symbols) and the average +SD (bars) of groups are represented (B,D,F,H). Statistical analysis was performed using the Mann Whitney test: n.s., non-significant.
