Figure 3
From: TRSP is dispensable for the Plasmodium pre-erythrocytic phase
Infectivity of TRSP- sporozoites through mosquito bite. Mosquitoes infected with TRSP- I21 (A,B) or I26 (C,D) and control parasites were used in bite experiments. Five to six mosquitos/mouse were allowed to probe for a total of 25 consecutive minutes. Parasite burden in the liver was assessed by live imaging on day 2 (D2) post-infection using IVIS Lumina LT. Bioluminescent signal was quantified and plotted as average radiance (photons/s/cm2/sr) (A,C). Starting on day 3 post-infection, parasitaemia of infected animals was determined daily by a Giemsa-stained blood smear (B,D). Values for individual mice (symbols) and the average +SD (bars) of groups are represented. Statistical analysis was performed using the Mann Whitney test: n.s., non-significant.
