Figure 6
From: Transposon mutagenesis screen in mice identifies TM9SF2 as a novel colorectal cancer oncogene
The Ets family transcription factor ELF1 regulates TM9SF2 expression. ELF1 is a potential regulator of TM9SF2 gene expression. (A) Position weight matrix from ENCODE CHIP-seq experiments showing a potential ELF1 transcription factor binding motif in the 5′ UTR of the TM9SF2 gene. (B) ELF1 and TM9SF2 mRNA co-expression scatter plot using TCGA RNAseq data from CRC patients. (C) qRT-PCR analysis for ELF1 expression in human CRC cell lines. HCEC, an immortalized but non-transformed colon epithelial cell line, was used as the control. (D) Data from ELF1 ChIP normalized against those from IgG mock ChIP. Schematic shows binding location of two qPCR primer sets (black and gray arrows) used to quantify TM9SF2 promoter DNA after ELF1 ChIP. Translation start site is indicated with bent arrow. t-test, ns = not significant, *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001.
