Figure 6
Ex vivo analysis of resected tumor tissue. Images of (a) caspase 3 and (b) GADD34 expression at 72 hours following dosing with vehicle, 8 mg kg−1, 9.5 mg kg−1 or 11 mg kg−1. Caspase 3-positive nuclei are stained brown (as labeled with a red arrow) and blue otherwise (haematoxylin), and example necrotic areas are labeled with a yellow arrow. GADD34-positive areas are stained brown and nuclei are counter-stained in blue with haematoxylin. (c) The results of Bradford assay, to measure gross tissue protein. Summary data from quantitative analysis of immunohistochemistry images are shown for caspase 3 (d) and GADD34 (e), for each study group. The scale bar in (a and b) represents 100 μm. Data points are mean ± standard error (*P < 0.05, **P < 0.01, ordinary one-way ANOVA of Mann-Whitney test).
