Figure 4
Effect of OCLN overexpression on cytotoxicity of anticancer drugs. (A) Mock and OCLN-expressing cells were treated with CDDP or DXR for 48 h. Cell viability was measured by WST-1 assays. (B) The expression of ABCB1, ABCC1, ABCC2, ABCG2, and β-actin in cell lysates was examined by western blotting. The protein levels of ABC transporters are shown as a percentage of the values in mock cells. The full-length blot images are shown in Supplementary Fig. S4. (C) The mRNA levels of ABCB1, ABCC1, ABCC2, ABCG2 are shown as a percentage of the values in mock cells. n = 3–6. NS, P > 0.05 compared with mock cells.
