Figure 3 | Scientific Reports

Figure 3

From: Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice

Figure 3

H2 production in mice reflects starch digestibility. Female (a) and male (d) mice were fed either HDD or LDD for three weeks and volume of H2 produced (VH2) was recorded for 24 h in the adapted indirect calorimetry system. Cumulative H2 production in females (b) and males (e) quantified during the 12 h light phase (LP), 12 h dark phase (DP) or the complete 24 h photoperiod. Cumulative starch and total food intake in females (c) and males (f) over the measuring period calculated from food intake records. White and grey areas represent the light and the dark phase, respectively. Time course data was analysed by repeated measures two-way ANOVA with Bonferroni’s test for multiple comparisons and time points where P < 0.05 are indicated with black asterisks (panels a and d). Other statistical comparisons made by Student’s t-test or Mann-Whitney U test; *P ≤ 0.05, ****P < 0.0001 (n = 11 LDD females, n = 12 remaining groups). Data shown as mean ± s.d. ZT, Zeitgeber time.

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