Figure 2

Temporal expression of circadian clock genes in the SCN. (A) 24-hour temporal expression of Nr1d1, Per2, and BMAL1 mRNA measured in situ in the SCN relative to a region of similar size, dorsal and lateral to the SCN. Assessments were relative to GAPDH. N/P and B/P represent the within subject expression ratios of Nr1d1/Per2 and BMAL1/Per2, respectively. Error bars = SEM. Data are from C57Bl6 mice; n = 5 per group. (B–D) Relative mRNA expression of Nr1d1, Per2, and N/P ratio at ZT04 and ZT11 in two control groups, using two mRNA quantification methods. P values are based on individual t-tests. N = 4/group.