Figure 4
Effects of behavioral conditioning on temporal gene expression. Nr1d1, Per2 and N/P ratio is shown in four selected tissues. Each graph represents two conditioning experiments comparing gene expression following place conditioning at either ZT03 or ZT11 with procedural controls. Animals were tested for place preference at either ZT03 or ZT11. Tissues were obtained one hour after testing. Phase dependent differences within each experimental condition (control, ZT03 conditioned, ZT11 conditioned) were evaluated using planned comparisons and t-tests [*p < 0.05; **p < 0.01, ***p < 0.001]. Effects of conditioning were evaluated using one-way ANOVAs for control vs. ZT03 conditioning [aap < 0.01] and vs. ZT11 conditioning [bp < 0.05; bbp < 0.01]). Alteration in the relative expression at ZT03 vs. ZT11 were determined from difference scores between the two times [#p < 0.001]. P values for comparisons of all difference scores are shown in Table 1. Error bars = SEM.
