Figure 2 | Scientific Reports

Figure 2

From: Plasmid-normalized quantification of relative mitochondrial DNA copy number

Figure 2

Standard curve regression of a DNA template (A) and plasmid DNA (B). Quantification cycle (Cq) values on the y-axis and serial 3-fold dilution of the DNA template (A) and plasmid DNA (B) on the x-axis assayed in quadruplicate by qPCR. Equation of linear regression, R2 and efficiencies of both targets are described in the tables. Standard curve of the calibrator plasmid (containing both targets in a 1:1 ratio) revealed differences in emission intensities of the fluorescent dyes Yakima Yellow and FAM.

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