Figure 7
From: Plasmid-normalized quantification of relative mitochondrial DNA copy number
Chart showing mtDNA content measured in 45 identical aliquots obtained from the same blood sample extracted in parallel using three different DNA extraction methods. DNA was extracted using EZ1 DNA Blood 200 µl kit (EZ1), PureGene kit (PG) and Chelex resin (CH). Higher values and considerable variability were detected with the two manual methods (INV 332.1 ± 130.3 and Chelex 206.5 ± 55.2 mtDNA copies, average CV were 27.5% and 24.6% respectively) than in the automated method EZ1 (averaged 63.8 mtDNA copies ±15.7, average CV was 15%). The quantitative mtDNA content is shown on the y-axis.
