Figure 3
Establishing the target identification scoring system. (a) Schematic display of the generation of specific target pattern using E2-P1283 as model. The blue line is the mean of target profiles. The orange line is a profile of test sample, E18-P1283. The gray zone represents identification tolerance boundary. The red area represents major differences between target and test sample. The TIS formula is shown in the bottom left. (b,c) TIS system was validated by using a training set. Isolates E2 and E18 were used to assess target identification accuracy by 2 different primers, (b) P1254 and (c) P1283. The TIS thresholds for each test are shown in parentheses. Numbers highlighted in red indicate instances where the TIS was less than the threshold, in which the corresponding samples were matched to the target cluster.
