Figure 6
From: A Genome-wide View of Transcriptome Dynamics During Early Spike Development in Bread Wheat
Expression pattern of putative TaSVP, TaLAX1 and TabHLH27. (A,C,E) RT-qPCR validation of the expression patterns at six developmental stages for two homologous genes of putative TaSVP (A), TaLAX1 (C) and TabHLH27 (E). FPKM: normalized expression levels extracted from RNA-seq data. qPCR: transcript levels measured by real-time RT-PCR. TaACTIN served as the internal control. Three biological replicates were performed. Error bars represent SD from three biological repeats. The maximum expression level was set as “1”. (B) Expression patterns of putative TaSVP by in situ hybridization with antisense probe. Signals were detected in inflorescence meristems (red arrows) but not floral meristems (blue arrows). (D) Expression patterns of putative TaLAX1 by in situ hybridization with antisense probe. Signals were detected at axillary meristem generating sites and indicated by red arrows. (E) Expression patterns of TabHLH27 by in situ hybridization with antisense probe. Signals in leaf primordia were indicated by blue arrows and signals in meristem were indicated by red arrows. Bar: 100 µm in (D,E,I,J). Developmental stages were indicated.
