Figure 3
From: Dual Inhibition of Bcl-2/Bcl-xL and XPO1 is synthetically lethal in glioblastoma model systems
The combination treatment of ABT263 and selinexor elicits apoptosis in a partial caspase-dependent manner. (A) LN229, T98G and U87 GBM cells were treated with ABT263, selinexor or the combination. Thereafter, whole-cell protein lysates were harvested and analyzed by conventional western blotting for the expression of PARP, caspase-9 (CP9), cleaved caspase-3 (cCP3) and Actin. TF: total form, CF: cleaved form. (B) T98G cells were treated with vehicle or the combination treatment of ABT263+ selinexor in the presence or absence of pan-caspase inhibitor, zVAD-fmk. Thereafter, cells were fixed, stained with propidium iodide and analyzed by flow cytometry. Shown are representative flow plots. (C) The experiment in B was quantified and statistical analysis was performed. (C) Control, AS: ABT263+ Selinexor, Z: zVAD-fmk, ZAS: ZVAD-fmk + ABT263+ Selinexor. Shown are means and SD. *p < 0.05; **/***/****p < 0.01.
