Figure 3 | Scientific Reports

Figure 3

From: Development of a high sensitivity TaqMan-based PCR assay for the specific detection of Mycobacterium tuberculosis complex in both pulmonary and extrapulmonary specimens

Figure 3

DMSO enhanced qPCR performance. (a) The IS4 amplicons were amplified in the presence of BSA (0.8 μg/μl), Glycerol (4%) or DMSO (4%), respectively (initial DNA: 10 copies). The figure listed the average, standard deviation (STD) of Ct value for each treatment. The average Ct values and STD were calculated on the basis of twelve replicates. The average Ct of DMSO treatment was significantly lower than the other treatments. (b) IS4 qPCR assay was able to detect 10 copies of IS6110 synthetic vectors in the presence of different additives. The percentage was shown in the bars. Positive rate means PCR amplification successfully. Negative rate means not detected.

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