Figure 4 | Scientific Reports

Figure 4

From: Development of a high sensitivity TaqMan-based PCR assay for the specific detection of Mycobacterium tuberculosis complex in both pulmonary and extrapulmonary specimens

Figure 4

Primer optimization. Various combinations of primers were tested, (a) by using 100 copies of DNA amount: combination composed of F700 (Forward primer, 700 nM) & R350 (Reverse primer, 350 nM) attained significantly lowest Ct value. (b) by using 1,000 copies of DNA amount: combination composed of F700 (Forward primer, 700 nM) & R350 (Reverse primer, 350 nM) attained significantly lowest Ct value. (c) by using 10,000 copies of DNA amount: all the primer combinations generated similar Ct values except F175 combinations, whose forward primer concentration was 175 nM.

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