Table 1 miRNAs identified by the NanoString nCounter miRNA expression assay in human cumulus granulosa cells. Forty-three of the miRNAs were differentially expressed with a fold change >2 and an FDR < 5%.

From: Characterization of the miRNA regulators of the human ovulatory cascade

miR	logFC CCM2 vs. CCGV	Average Expression CCGV	Average Expression CCM2	p-value	Adj. p-value	Putative granulosa cell function	References
hsa-let-7a-5p*	3.81363	3642.69	43742.2	0.000154	0.000256	Apoptosis, cumulus vs. mural	^{8, 50, 63}
hsa-let-7b-5p	3.30288	2591.97	20276.4	0.000497	0.000693
hsa-let-7c	3.87105	506.408	6725.24	3.53E-06	2.91E-05
hsa-let-7d-5p	3.10472	376.498	2855.3	3.91E-05	0.000106
hsa-let-7e-5p	3.54614	491.637	5066.29	1.15E-05	4.41E-05
hsa-let-7f-5p*	2.66036	348.055	2230.98	0.000156	0.000256
hsa-let-7g-5p*	4.17388	1762.22	31204.5	1.99E-05	6.11E-05
hsa-let-7i-5p	3.2881	279.589	2871.92	7.02E-06	0.000036
hsa-miR-106a-5p/ miR-17-5p	1.89582	1418.37	4268.29	0.017636	0.019316	Bovine follicle development	⁶⁴
hsa-miR-107	2.20654	289.935	1226.41	0.000389	0.00056	Bovine follicle development, Steroidogenesis	^{65, 66}
hsa-miR-125a-5p	3.81061	2536.53	30936.3	9.05E-05	0.000189	Apoptosis	⁶⁷
hsa-miR-125b-5p	5.00927	2516.64	70782.3	3.79E-06	2.91E-05	Bovine follicle development, Steroidogenesis, cumulus vs. mural	^{8, 65, 66}
hsa-miR-130a-3p	3.27081	559.043	4177.33	5.68E-05	0.000131	Bovine follicle development, PCOS	^{66, 68}
hsa-miR-132-3p	5.59724	290.378	15338.4	3.9E-09	8.96E-08	cumulus vs. mural, Steroidogenesis, PCOS, murine follicle development	^{8, 11, 68}
hsa-miR-15a-5p	3.15798	1280.84	10868	0.000342	0.000507	Steroidogenesis	⁶⁵
hsa-miR-15b-5p	2.42639	1952.83	8482.74	0.004694	0.005682	Steroidogenesis	⁶⁵
hsa-miR-16-5p	3.5448	1493.38	16847.5	0.000103	0.000206	Bovine follicle development	⁸
hsa-miR-181a-5p	3.82643	404.396	5005.58	7.08E-06	0.000036	cumulus vs. mural, proliferation	^{8, 69}
hsa-miR-191-5p*	2.80069	1557.66	10258.1	0.001191	0.001565	Bovine follicle development, cumulus vs. mural	^{8, 70}
hsa-miR-19a-3p	3.09878	381.085	2980.11	4.71E-05	0.000114	Bovine follicle development, Steroidogenesis, PCOS	^{64,65,– 66, 68}
hsa-miR-19b-3p	3.33449	1177.47	10517.6	0.000143	0.000253	Ovarian hyperstimulation	⁷¹
hsa-miR-20a-5p/ miR-20b-5p	3.85536	1094.21	16458.7	2.87E-05	8.26E-05	cumulus vs. mural	⁸
hsa-miR-21-5p*	8.54162	21.5677	6740.8	1.69E-11	7.79E-10	cumulus vs. mural, murine follicle development, apoptosis	^{8, 11, 40}
hsa-miR-223-3p	2.58013	254.152	1404.38	0.000114	0.000216	cumulus vs. mural, Steroidogenesis, PCOS	^{8, 72}
hsa-miR-22-3p*	1.75262	1807.13	4565.75	0.041854	0.044774	cumulus vs. mural, Steroidogenesis, PCOS	^{8, 72}
hsa-miR-23b-3p	2.59206	281.984	1384.31	0.000117	0.000216	Bovine ovary	⁷³
hsa-miR-25-3p	2.06898	1683.34	7156.33	0.013105	0.014703	Steroidogenesis	⁶⁵
hsa-miR-29a-3p	4.94371	536.265	13587.1	5.05E-07	7.74E-06	Steroidogenesis	⁷⁴
hsa-miR-29b-3p	3.91754	672.73	7187.08	0.000016	5.26E-05	Steroidogenesis, cumulus vs. mural	^{8, 65}
hsa-miR-302d-3p	−0.25	1660.7	1305.76	0.710	0.710**	n/a
hsa-miR-34a-5p	3.09029	245.28	2018.34	8.49E-06	0.000036	Apoptosis	^{55, 75}
hsa-miR-361-5p	3.15727	329.908	2675.28	1.59E-05	5.26E-05	Murine follicle development	¹¹
hsa-miR-374a-5p	2.05355	841.464	2757.13	0.007376	0.008482	cumulus vs. mural,	⁸
hsa-miR-378e	−1.3021	3643.64	2397.74	0.13083	0.13678**	Steroidogenesis	⁷⁶
hsa-miR-424-5p	4.68931	1405.72	27294.5	7.48E-06	0.000036	Age	¹⁷
hsa-miR-4454	3.79594	2852.29	62869.6	0.000325	0.000498	n/a
hsa-miR-450a-5p	2.70382	1349.62	8663.35	0.001472	0.001881	Bovine follicle development	⁶⁶
hsa-miR-451a*	1.218	1669.72	3424.96	0.176	0.180**	cumulus vs. mural	⁸
hsa-miR-503	4.2726	750.23	13810.1	2.66E-06	2.91E-05	Bovine follicle development,	^{12, 68, 77}
hsa-miR-508-3p	3.27969	208.958	2839.37	8.61E-06	0.000036	PCOS	¹⁶
hsa-miR-514a-3p	2.63133	282.897	2477.97	0.000185	0.000294	PCOS	¹⁶
hsa-miR-514b-5p	1.8413	431.937	1844.75	0.005467	0.006449	PCOS	⁷²
hsa-miR-720	3.10848	1958.04	22615.3	0.001832	0.002277	PCOS, Age	^{16, 17}
hsa-miR-93-5p	3.30979	620.555	5549.2	0.000045	0.000114	Proliferation, PCOS	^{78, 79}
hsa-miR-99a-5p*	2.89148	1585.53	10215.6	0.000935	0.001266	cumulus vs. mural, Bovine follicle development	^{8, 70, 80}
hsa-miR-99b-5p	2.74138	357.19	2252.67	7.72E-05	0.000169	Age, Bovine follicle development, PCOS	^{64, 67, 81}

*miRNA detected as one of the 10 most abundant in human cumulus cells⁸.
**Differential expression between CCGV and CCM2 samples was not significant.

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Table 1 miRNAs identified by the NanoString nCounter miRNA expression assay in human cumulus granulosa cells. Forty-three of the miRNAs were differentially expressed with a fold change >2 and an FDR < 5%.

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