Figure 5
Expression of KRAS G12V alone or in combination with p16 loss and small-T-antigen expression in U57810 cells does not change Yap/Taz transcriptional activity. (A) Representative Western blots showing total Yap, Taz, and phosphorylated Yap levels in U57810 cells, with Gapdh as loading control. (B) Effect of KRAS G12V expression on 8xGTIIC Yap/Taz Tead1-4 luciferase reporter normalised to Tk-renilla in U57810 cells. Data is presented as fold change compared to control (mean ± SD) from independent experiments. (C) Representative Western blots showing total Yap, Taz, and phosphorylated Yap S112/Taz S89 levels in U57810 cells, with Gapdh as a loading control. (D) Effect of KRAS G12V expression combined with p16 loss and small-T-antigen expression on 8xGTIIC Yap/Taz Tead1-4 luciferase reporter normalised to Tk-renilla in U57810 cells. Data is presented as fold change compared to control (mean ± SD) from 3 independent experiments.
