Figure 1 | Scientific Reports

Figure 1

From: Pre-B acute lymphoblastic leukemia expresses cell surface nucleolin as a 9-O-acetylated sialoglycoprotein

Figure 1

CCA lectin chromatography identifies a 100-kDa protein in pre-B ALL cells as NCL. (a) Image of silver-stained SDS-PAA gel showing different column fractions of a US7 pre-B ALL cell lysate. M = marker; Wh = whole cell lysate; Un = flow-through from CCA lectin affinity column; W = wash; E = eluate. The boxed area indicates the band analyzed by mass spectrometry. Table: Characteristics of 100 kDa NCL band analyzed by mass spectrometry (the 58.5 kDa CRA_c nucleolin isoform has 100% sequence identity with the 75 kDa CRA_b isoform). (b,c) Western blot analysis (b) of different pre-B ALLs as indicated in (c) using antibody against NCL. PDX, patient-derived xenograft.

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