Figure 1
A schematic representation of a 2D cross-section of intracellular delivery using a thermoplamonic substrate in an aqueous environment. (a) A cell seeded onto metallic micropyramids surrounded by a solution containing delivery payload. (b) The whole system is irradiated with a large-area pulsed laser beam from above, resulting in the localized formation of explosive boiling and bubbles at each pyramid. (c) The bubbles induce pores in the cell membrane. (d) Delivery payload enters the cell through the pores. (e) An inset from (d) shows payload entering the cell. The composition of the pyramids is also shown, which include a polymer substrate and thin metallic coating. (f) The cell membrane heals and the payload is retained in the cytosol.
