Figure 2
Novel AGE modification sites on human cardiac troponin-T (TnT). (A) MS/MS spectrum of trypsin-digested TnT unglycated peptide 227–240 (m/z 832.461). (B) MS/MS spectrum of trypsin-digested TnT peptide 227–240 with CML modification (+29 m/z = +58 Da) of Lys227 (m/z 861.464), confirmed by 4 peptides. (C) MS/MS spectrum of trypsin-digested TnT unglycated peptide 124–134 (m/z 666.374). (D) MS/MS spectrum of trypsin-digested TnT peptide 124–134 with CML modification (+29 m/z = +58 Da) of Lys125 (m/z 695.377), confirmed by 3 peptides. The b-ions denote N-terminal ions and y-ions denote C-terminal ions. CML, N ε-carboxymethyl-lysine.
