Table 2 Identification of phosphorylation sites on human cardiac troponin-I using LC-MS/MS analysis.

From: Cardiac troponins may be irreversibly modified by glycation: novel potential mechanisms of cardiac performance modulation

Peptide Sequence

Phosphorylated Residue

# Phosphorylated Peptides

Calculated Mol. Mass

Observed m/z

Observed Mol. Mass

Error ppm

Identity Score

Expect

Charge

21RRSSNYR27

S23/24

2

1097.4168

549.7153

1097.4160

−0.76

44

3.5E-03

+2

549.7153

1097.4160

−0.71

41

6.9E-03

+2

46KLQLKTLLLQIAK58

T51

3

1588.9681

795.4868

1588.9591

−5.65

54

7.7E-05

+2

530.6605

1588.9596

−5.32

46

4.2E-04

+3

795.4870

1588.9595

−5.37

32

1.2E-02

+2

51TLLLQIAKQELER63*

1

1633.8804

817.9432

1633.8719

−5.17

50

2.3E-03

+2

118VTKNITEIADLTQK131

T119

3

1652.8386

827.4221

1652.8296

−5.41

79

7.6E-06

+2

827.4241

1652.8336

−3.03

78

1.1E-05

+2

827.4225

1652.8305

−4.87

86

6.7E-07

+2

121NITEIADLTQKIFDLR136*

T123

1

1968.9921

985.5000

1968.9854

−3.39

74

2.2E-05

+2

146RVRISADAMMQALLGAR162

S150

2

1937.9692

647.0000

1937.9782

4.62

54

3.6E-03

+3

646.9972

1937.9697

0.24

59

8.3E-04

+3

147VRISADAMMQALLGAR162

6

1797.8630

891.9428

1781.8711

−0.17

76

2.4E-05

+2

594.9632

1781.8678

1.65

48

9.6E-02

+3

594.9639

1781.8698

1.22

79

1.3E-05

+3

891.9424

1781.8703

0.95

44

3.9E-02

+2

891.9436

1781.8727

2.54

65

3.2E-04

+3

594.9645

1781.8715

1.92

53

5.3E-03

+2

149ISADAMMQALLGAR162

3

1526.6986

764.3565

1526.6985

−0.08

95

1.3E-07

+2

764.3567

1526.6988

0.15

92

1.2E-07

+2

764.3565

1526.6985

−0.09

62

1.3E-04

+2

163AKESLDLR170

S166

2

1010.4797

506.2469

1010.4792

−0.48

54

2.8E-03

+2

506.2469

1010.4793

−0.45

41

5.0E-02

+2

194NIDALSGMEGR204*

S199

1

1257.5060

629.7601

1257.5056

−0.31

33

6.4E-02

+2

  1. S, serine; T, threonine; *Indicates single detection event data.
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