Figure 5

R430 suppresses EGFP expression in a transient plasmid transfection assay. Vero cells were transfected with pEGFP-N1. Two hours after the transfection cells were treated with R430 (10 µM) or ACV (50 µM). The corresponding EGFP expression under different conditions is depicted (a). The percentage of EGFP-positive cells was measured 24 hours after the transfection by flow cytometry (b).