Figure 5

Effects of 8 weeks of diet and genotype on hypothalamic Serpina3n and Socs3 expression. (A) In situ hybridization expression of Serpina3n in the hypothalamus of LFD and HFD fed Wild-Type mice at 3 days exposure; (B) In situ hybridization expression of Socs3 in the hypothalamus of LFD and HFD fed Wild-Type mice at 7 days exposure; (C) In situ hybridization of Socs3 expression in the arcuate nucleus (ARC) of Wild-Type mice, (D) Tlr4^−/− mice, and (E) Cd14^−/− mice; (F) In situ hybridization of Serpina3n expression in the arcuate nucleus and dorsal medial hypothalamus of Wild-Type, (G) Tlr4^−/−, and (H) Cd14^−/− mice; n = 6–9 mice/group (n = 3–4 mice/group for Tlr4^−/−); Data are mean ± SEM, circles indicate individual mice. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.